circRNA basic information
circBase ID: hsa_circ_0000376
Name: -
Synonym: -
Host Gene: -
Genomic location(hg19): chr12:11199618-11248400:-
Genomic location(hg38): chr12:11047019-11095801:-
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0001056
MONDO name: gastric cancer
Disease details: gastric cancer
Disease DO ID:
 10534
Disease MeSH ID:
 -
Disease NCIt ID:
 C9331
Disease ICD11 ID:
 1397617262
Disease OMIM ID:
 613659
Species: Human
Species details: Homo sapiens
Tissue specimen:

-
Cell lines:

AGS; HGC27
In vivo animal model:

-
circRNA-disease information
Expression pattern:
UN
Associated gene: GLUT1, LDHA
Associated microRNA: miR-145-5p
Biological function: Promotes viability, migration, invasion and glycolysis; inhibits apoptosis of gastric cancer cells.
Molecular mechanism: Acts as a sponge of miR-145-5p; negatively regulates miR-145-5p; involved in bupivacaine anti-tumor effects via circ_0000376/miR-145-5p axis.
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); glycolysis (promotes); ceRNA regulation (promotes)
Detected method:
Q
Validation methods:

RT-qPCR; Bioinformatics Analysis; Luciferase Reporter Assay; Transfection; CCK8; Transwell Assay; Annexin V/PI Flow Cytometry; Western Blot
Clinical significance:

-
Description:

In AGS and HGC27 gastric cancer cells, circ_0000376 promotes malignant phenotypes (viability, migration/invasion, glycolysis) and suppresses apoptosis. Mechanistically, circ_0000376 sponges miR-145-5p and negatively regulates its level; bupivacaine decreases circ_0000376, thereby increasing miR-145-5p and inhibiting malignant behaviors.
Confidence score:

0.5607
Other information
Title:

Bupivacaine suppresses the progression of gastric cancer through regulating circ_0000376/miR-145-5p axis.

Journal: BMC anesthesiology
Published: 2020
PubMed ID: 33126850
Study type:

biological research
Data availability: The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.
Code availability: -