| Expression pattern: |
DN |
| Associated gene: |
HIPK2 |
| Associated microRNA: |
miR-147b |
| Biological function: |
Suppresses PDAC cell proliferation and glycolysis and enhances apoptosis; inhibits tumorigenesis in vivo; exosomal hsa_circ_0012634 represses PDAC cell behaviors. |
| Molecular mechanism: |
Acts as a miRNA sponge for miR-147b to upregulate HIPK2 (ceRNA axis), thereby suppressing PDAC progression; exosome-transmitted hsa_circ_0012634 exerts similar effects. |
| Biological pathway or process: |
proliferation (inhibits); apoptosis (promotes); glycolysis (inhibits); ceRNA regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; Nuclear-Cytoplasmic Fractionation; RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Colony Formation Assay; Annexin V/PI Flow Cytometry; Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry); ROC Analysis; Bioinformatics Analysis; Clinical Sample Validation |
| Clinical significance: |
Low expression in serum exosomes of PDAC patients; ROC AUC 0.868 suggests diagnostic potential; proposed diagnosis and treatment biomarker for PDAC. |
| Description: |
hsa_circ_0012634 is down-regulated in PDAC tissues/cells and in serum exosomes from PDAC patients. Functionally, its overexpression (including exosome-delivered form) inhibits PDAC proliferation and glycolysis and promotes apoptosis by sponging miR-147b to derepress HIPK2, reducing tumor growth in a PANC-1 xenograft model and showing diagnostic potential by ROC analysis. |
| Confidence score: |
0.8657 |