| Expression pattern: |
DN |
| Associated gene: |
PTEN |
| Associated microRNA: |
miR-130b, miR-494 |
| Biological function: |
inhibits migration; inhibits invasion; inhibits proliferation; suppresses tumor growth |
| Molecular mechanism: |
circSLC8A1 directly binds miR-130b and miR-494 and acts as a miRNA sponge to regulate PTEN expression and downstream PI3K/Akt signaling. |
| Biological pathway or process: |
migration (inhibits); invasion (inhibits); proliferation (inhibits); PI3K/AKT (inhibits); ceRNA regulation (promotes) |
| Detected method: |
Q
S
|
| Validation methods: |
RNA-seq; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; RNA Pull-Down; Luciferase Reporter Assay; FISH / smFISH; Transfection; CCK8; Wound Healing Assay; Transwell Assay; Western Blot; IHC (Immunohistochemistry); In Vivo Animal Model; H&E Staining; Bioinformatics Analysis; Clinical Sample Validation |
| Clinical significance: |
circSLC8A1 expression was associated with the pathological stage and histological grade of bladder cancer; may provide a potential biomarker and therapeutic target for the management of bladder cancer. |
| Description: |
In bladder cancer, circSLC8A1 is down-regulated and functions as a tumor suppressor. It sponges miR-130b and miR-494 to de-repress PTEN, thereby inhibiting PI3K/Akt-related signaling and reducing proliferation, migration, invasion, and xenograft growth. |
| Confidence score: |
0.8634 |