| Expression pattern: |
DN |
| Associated gene: |
CDX1, Hu antigen R (HuR), RAB36 |
| Associated microRNA: |
miR-155-5p |
| Biological function: |
Inhibits metastatic potential (invasion and migration) and suppresses liver metastasis of CRC. |
| Molecular mechanism: |
Acts as a sponge/decoy for miR-155-5p to upregulate CDX1; sequesters HuR to reduce HuR-mediated stabilization of RAB36 mRNA, decreasing RAB36. |
| Biological pathway or process: |
migration (inhibits); invasion (inhibits); metastasis (inhibits); ceRNA regulation (promotes); mRNA stability (inhibits) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Sanger Sequencing; Nuclear-Cytoplasmic Fractionation; ISH (In Situ Hybridization); RNA Pull-Down; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; Transwell Assay; In Vivo Animal Model; Western Blot; Clinical Sample Validation |
| Clinical significance: |
Potential clinical diagnosis/therapeutic target for liver metastatic CRC. |
| Description: |
circPPFIA1-S (hsa_circ_0000337) is downregulated in liver-metastatic CRC models and patient liver metastatic tissues. It suppresses invasion/migration and liver metastasis by sponging miR-155-5p to increase CDX1, and by binding/sequestering HuR to reduce HuR-driven RAB36 mRNA stabilization and expression. |
| Confidence score: |
0.8439 |