circRNA basic information
circBase ID: hsa_circ_0000284
Name: hsa_circ_HIPK3
Synonym: circHIPK3 / Circular RNA HIPK3
Host Gene: HIPK3
Genomic location(hg19): chr11:33307958-33309057:+
Genomic location(hg38): chr11:33286412-33287511:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0004986
MONDO name: urinary bladder carcinoma
Disease details: bladder cancer / BCa
Disease DO ID:
 4007
Disease MeSH ID:
 -
Disease NCIt ID:
 C4912
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

bladder cancer tissues; tumor-adjacent tissues; tumor tissues
Cell lines:

SV-HUC-1; T24; EJ
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
DN
Associated gene: VCP, Beclin 1, ataxin-3
Associated microRNA: miR-558
Biological function: Inhibits bladder cancer cell proliferation and suppresses autophagy.
Molecular mechanism: circHIPK3 binds VCP and blocks the VCP/Beclin 1 interaction, thereby inhibiting VCP-mediated autophagy (non-classical ceRNA mechanism).
Biological pathway or process:

autophagy (inhibits); proliferation (inhibits); PI3K/AKT (other)
Detected method:
Q
Validation methods:

RT-qPCR; Clinical Sample Validation; FISH / smFISH; RIP (RNA Immunoprecipitation); RNA Pull-Down; Western Blot; Co-IP; IF (Immunofluorescence); Transfection; CCK8; EdU Staining; In Vivo Animal Model; IHC (Immunohistochemistry)
Clinical significance:

circHIPK3 was negatively correlated with bladder cancer grade, invasion, and lymph node metastasis; proposed as a biomarker for bladder cancer.
Description:

circHIPK3 (hsa_circ_0000284) is down-regulated in bladder cancer tissues and cell lines and acts as a tumor suppressor. It inhibits proliferation and autophagy by directly binding VCP and weakening the VCP-Beclin 1 interaction, reducing Beclin 1-associated autophagy activity.
Confidence score:

0.7788
Other information
Title:

CircHIPK3 negatively regulates autophagy by blocking VCP binding to the Beclin 1 complex in bladder cancer.

Journal: Discover oncology
Published: 2023
PubMed ID: 37269429
Study type:

combined biological and clinical study
Data availability: The datasets generated during and/or analysed during the current study are available from the corresponding author on reasonable request.
Code availability: -