| Expression pattern: |
DN |
| Associated gene: |
CREBZF, ADAR1, STAT3 |
| Associated microRNA: |
miR-1269a |
| Biological function: |
inhibits proliferation; promotes apoptosis; suppresses tumor growth |
| Molecular mechanism: |
ADAR1 binds flanking intron complementary sequences to suppress circPAPD4 circularization; circPAPD4 sponges miR-1269a to increase CREBZF; CREBZF suppresses STAT3 dimerization and ADAR1, forming a positive feedback loop (circPAPD4/miR-1269a/CREBZF/STAT3/ADAR1). |
| Biological pathway or process: |
proliferation (inhibits); apoptosis (promotes); ceRNA regulation (other); JAK/STAT (inhibits) |
| Detected method: |
Q
H
M
|
| Validation methods: |
Microarray; Bioinformatics Analysis; RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; FISH / smFISH; Sanger Sequencing; ISH (In Situ Hybridization); Clinical Sample Validation; Survival Analysis; Pearson correlation analysis; RNA Pull-Down; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Transfection; CCK8; EdU Staining; Annexin V/PI Flow Cytometry; TUNEL; Western Blot; Co-IP; In Vivo Animal Model; IF (Immunofluorescence); H&E Staining |
| Clinical significance: |
Low circPAPD4 expression was substantially correlated with larger tumor size, advanced tumor-node-metastasis (TNM) stages, and higher Ki-67 expression of BC tumors; patients with lower circPAPD4 expression presented an unfavorable RFS; circPAPD4 expression served as an independent prognostic factor for RFS in BC. |
| Description: |
circPAPD4 is down-regulated in breast cancer and functions as a tumor suppressor by inhibiting proliferation and promoting apoptosis. It mainly localizes to the cytoplasm and acts as a ceRNA by sponging miR-1269a to increase CREBZF, which inhibits STAT3 dimerization to reduce ADAR1 and thereby enhances circPAPD4 biogenesis in a positive feedback loop. |
| Confidence score: |
0.8941 |