circRNA basic information
circBase ID: hsa_circ_0005199
Name: hsa_circ_UBE4B
Synonym: circUBE4B
Host Gene: UBE4B
Genomic location(hg19): chr1:10155518-10165802:+
Genomic location(hg38): chr1:10095460-10105744:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005580
MONDO name: esophageal squamous cell carcinoma
Disease details: esophageal squamous cell carcinoma / ESCC
Disease DO ID:
 3748
Disease MeSH ID:
 C562729; D000077277
Disease NCIt ID:
 C4024
Disease ICD11 ID:
 1417891145
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

ESCC tissue; paired adjacent non-cancerous tissues
Cell lines:

TE1; Eca109; KYSE-150; KYSE-180; HEEC; HEK293T
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: MAPK1
Associated microRNA: -
Biological function: promotes proliferation; promotes tumor growth/tumorigenicity
Molecular mechanism: circUBE4B encodes a protein (circUBE4B-173aa) that binds MAPK1 and increases MAPK1 phosphorylation, activating MAPK/ERK signaling.
Biological pathway or process:

proliferation (promotes); cell cycle (promotes); MAPK (promotes); ERK (promotes)
Detected method:
Q
M
Validation methods:

Microarray; RT-qPCR; RNase R Treatment; FISH / smFISH; Actinomycin D / DRB Stability Assay; divergent primers PCR; Sanger Sequencing; Transfection; CCK8; Colony Formation Assay; EdU Staining; Cell Cycle Assay; In Vivo Animal Model; Survival Analysis; Clinical Sample Validation
Clinical significance:

circUBE4B expression levels were associated with tumor size and differentiation and high circUBE4B predicted worse prognosis (shorter OS and PFS).
Description:

circUBE4B (hsa_circ_0005199) is upregulated in ESCC and promotes ESCC cell proliferation and xenograft growth. Its tumor-promoting effect depends on translation of circUBE4B-173aa, which binds MAPK1, increases MAPK1 phosphorylation, and activates MAPK/ERK signaling; high circUBE4B is associated with worse patient survival.
Confidence score:

0.7964
Other information
Title:

A novel protein encoded by circUBE4B promotes progression of esophageal squamous cell carcinoma by augmenting MAPK/ERK signaling.

Journal: Cell death & disease
Published: 2023
PubMed ID: 37264022
Study type:

combined biological and clinical study
Data availability: GSE131969; Supplementary Material 2
Code availability: Table S9