circRNA basic information
circBase ID: hsa_circ_0026337
Name: hsa_circ_SCN8A
Synonym: circSCN8A
Host Gene: SCN8A
Genomic location(hg19): chr12:52180325-52188425:+
Genomic location(hg38): chr12:51786541-51794641:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005233
MONDO name: non-small cell lung carcinoma
Disease details: non-small cell lung cancer / NSCLC
Disease DO ID:
 3908
Disease MeSH ID:
 D002289
Disease NCIt ID:
 C2926
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

tumor tissues; paired paracancerous tissues; adjacent non-cancerous tissues; subcutaneous xenograft tumors
Cell lines:

BEAS-2B; A549; H1299; NCI-H520; SK-MES-1
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
DN
Associated gene: ACSL4, SCN8A, SLC7A11, GPX4, Ki-67, E-cadherin, N-cadherin, Vimentin
Associated microRNA: miR-1290
Biological function: CircSCN8A suppresses NSCLC cell proliferation, migration, invasion, EMT, metastasis and tumor growth, and promotes ferroptosis.
Molecular mechanism: CircSCN8A acts as a ceRNA by sponging miR-1290 to up-regulate ACSL4 expression, thereby inducing ferroptosis and inhibiting malignant progression in NSCLC.
Biological pathway or process:

proliferation (inhibits); migration (inhibits); invasion (inhibits); metastasis (inhibits); EMT (inhibits); ferroptosis (promotes); ceRNA regulation (other)
Detected method:
Q
M
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; Microarray; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Colony Formation Assay; Transwell Assay; Wound Healing Assay; In Vivo Animal Model; ELISA; Western Blot; IHC (Immunohistochemistry); Cohort Study; Survival Analysis; Bioinformatics Analysis
Clinical significance:

Low circSCN8A expression was associated with aggressive clinicopathological characteristics and poor prognosis; circSCN8A may be a prognostic biomarker and therapeutic target for NSCLC.
Description:

CircSCN8A is down-regulated in NSCLC tissues and cells, and low expression is associated with aggressive clinicopathological features and poorer overall survival. Functionally, circSCN8A acts as a tumor suppressor by inhibiting proliferation, migration, invasion, EMT/metastasis and xenograft growth while promoting ferroptosis. Mechanistically, it sponges miR-1290 to enhance ACSL4 expression, forming a circSCN8A/miR-1290/ACSL4 ceRNA axis.
Confidence score:

0.8824
Other information
Title:

CircSCN8A suppresses malignant progression and induces ferroptosis in non-small cell lung cancer by regulating miR-1290/ACSL4 axis.

Journal: Cell cycle (Georgetown, Tex.)
Published: 2023
PubMed ID: 36482742
Study type:

combined biological and clinical study
Data availability: GSE112214; GSE158695; GSE101684; GSE32863; GSE33532; GSE19188; GSE85841; datasets available from the corresponding author on reasonable request
Code availability: -