| Expression pattern: |
UP |
| Associated gene: |
NCAPG2, AGO2, AKT, mTOR, E-cadherin, Snail, Vimentin, Ki67 |
| Associated microRNA: |
miR-188-3p |
| Biological function: |
Promotes tumor proliferation, migration, invasion, EMT and in vivo tumor growth; inhibits apoptosis; facilitates cell cycle progression. |
| Molecular mechanism: |
Acts as a ceRNA by sponging miR-188-3p to relieve repression of NCAPG2, thereby activating AKT/mTOR signaling and inducing EMT; effects are mTOR-dependent and can be antagonized by rapamycin. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); cell cycle (promotes); EMT (promotes); PI3K/AKT/mTOR (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
H
M
|
| Validation methods: |
Microarray; RT-qPCR; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; RNase R Treatment; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; Luciferase Reporter Assay; RNA Pull-Down; RIP (RNA Immunoprecipitation); Transfection; CCK8; EdU Staining; Colony Formation Assay; Wound Healing Assay; Transwell Assay; Annexin V/PI Flow Cytometry; Cell Cycle Assay; TUNEL; Western Blot; IHC (Immunohistochemistry); H&E Staining; In Vivo Animal Model; ROC Analysis; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
circ0001955 could sensitively discriminate CSCCs from noncancerous tissues; suggested as a valuable marker and independent prognostic factor. |
| Description: |
circ0001955 is upregulated in CSCC and promotes proliferation, migration/invasion, EMT, and xenograft tumor growth. It sponges miR-188-3p to upregulate NCAPG2 and activates the AKT/mTOR pathway; mTOR inhibition (rapamycin) counteracts circ0001955-driven effects. |
| Confidence score: |
0.8893 |