circRNA basic information
circBase ID: hsa_circ_0001238
Name: hsa_circ_CCDC134
Synonym: circCCDC134
Host Gene: CCDC134
Genomic location(hg19): chr22:42204878-42206295:+
Genomic location(hg38): chr22:41808874-41810291:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
0005233
MONDO name: non-small cell lung carcinoma
Disease details: non-small cell lung cancer / NSCLC
Disease DO ID:
3908
Disease MeSH ID:
D002289
Disease NCIt ID:
C2926
Disease ICD11 ID:
-
Disease OMIM ID:
-
Species: Human
Species details: Homo sapiens
Tissue specimen:

NSCLC tumor tissues; adjacent normal tissues; serum exosomes

Cell lines:

H1299; HCC827; PC9; A549; BEAS-2B

In vivo animal model:

cell line-derived xenograft

circRNA-disease information
Expression pattern:
UP
Associated gene: NFAT5
Associated microRNA: miR-625-5p
Biological function: promotes NSCLC cell growth, metastasis and glycolysis; inhibits apoptosis
Molecular mechanism: ceRNA mechanism: circCCDC134 sponges miR-625-5p to regulate NFAT5
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); glycolysis (promotes); apoptosis (inhibits); ceRNA regulation (promotes)

Detected method:
Q
Validation methods:

RT-qPCR; Nuclear-Cytoplasmic Fractionation; RNase R Treatment; Actinomycin D / DRB Stability Assay; Clinical Sample Validation; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Transfection; Colony Formation Assay; EdU Staining; Transwell Assay; Wound Healing Assay; Annexin V/PI Flow Cytometry; Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry)

Clinical significance:

circCCDC134 was highly expressed in the serum exosomes of NSCLC patients compared with healthy normal controls, suggesting exosomal circCCDC134 could act as a diagnostic indicator/target for NSCLC.

Description:

circCCDC134 is up-regulated in NSCLC tissues/cells and enriched in patient serum exosomes. Functionally, circCCDC134 promotes proliferation, migration/invasion, tumor growth, and glycolysis while inhibiting apoptosis by acting as a ceRNA that sponges miR-625-5p and derepresses NFAT5.

Confidence score:

0.8327

Other information
Title:

A novel molecular mechanism mediated by circCCDC134 regulates non-small cell lung cancer progression.

Journal: Thoracic cancer
Published: 2023
PubMed ID: 37231545
Study type:

combined biological and clinical study

Data availability: The analyzed data sets generated during the present study are available from the corresponding author on reasonable request.
Code availability: -