| Expression pattern: |
UP |
| Associated gene: |
STC1, IL-6, IL-6R, gp130, AGO2 |
| Associated microRNA: |
miR-144-5p |
| Biological function: |
promotes CSC-like properties, proliferation, migration, and metastasis in gastric cancer |
| Molecular mechanism: |
acts as a miRNA sponge for miR-144-5p to upregulate STC1 and activate IL-6/JAK2/STAT3 signaling |
| Biological pathway or process: |
IL-6/JAK2/STAT3 signaling (promotes); proliferation (promotes); migration (promotes); metastasis (promotes); stemness (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Sanger Sequencing; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; Colony Formation Assay; CCK8; Cell Cycle Assay; Transwell Assay; Sphere formation assays; IF (Immunofluorescence); Flow Cytometry（Non-apoptosis/cycle）; Western Blot; ELISA; Co-IP; In Vivo Animal Model; H&E Staining; Clinical Sample Validation; Cohort Study; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
high circUBA2 expression is associated with poor prognosis in GC and may be a prognostic biomarker |
| Description: |
In gastric cancer, circUBA2 is up-regulated and promotes proliferation, migration, metastasis and CSC-like properties. It functions as a ceRNA by sponging miR-144-5p to upregulate STC1, thereby activating IL-6/JAK2/STAT3 signaling; high circUBA2 indicates poor prognosis and may be a prognostic biomarker/therapeutic target. |
| Confidence score: |
0.8933 |