circRNA basic information
circBase ID: hsa_circ_0000064
Name: hsa_circ_B4GALT2
Synonym: circ_0000064
Host Gene: B4GALT2
Genomic location(hg19): chr1:44446997-44447136:-
Genomic location(hg38): chr1:43981325-43981464:-
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005016
MONDO name: diabetic kidney disease
Disease details: diabetic nephropathy
Disease DO ID:
 -
Disease MeSH ID:
 D003928
Disease NCIt ID:
 C84417
Disease ICD11 ID:
 615062102
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

kidney biopsy samples; kidney tissues
Cell lines:

HRMCs; 293 T
In vivo animal model:

-
circRNA-disease information
Expression pattern:
UP
Associated gene: WNT2B
Associated microRNA: miR-424-5p
Biological function: Circ_0000064 promotes high glucose-induced HRMC proliferation, cell cycle progression, inflammatory responses and extracellular matrix accumulation; circ_0000064 knockdown attenuates these diabetic nephropathy-related cell dysfunctions.
Molecular mechanism: Circ_0000064 acts as a miR-424-5p sponge to relieve miR-424-5p-mediated inhibition of WNT2B, thereby regulating HG-induced mesangial cell dysfunctions.
Biological pathway or process:

proliferation (promotes); cell cycle (promotes); inflammation (promotes); fibrosis (promotes); ceRNA regulation (promotes)
Detected method:
Q
Validation methods:

RT-qPCR; RNase R Treatment; Clinical Sample Validation; Transfection; CCK8; EdU Staining; Cell Cycle Assay; ELISA; Western Blot; Bioinformatics Analysis; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation)
Clinical significance:

Circ_0000064 was upregulated in kidney biopsy samples from DN patients and was proposed as a biomarker in DN management.
Description:

In this study, circ_0000064 was upregulated in HG-treated human renal mesangial cells and in kidney biopsy samples from DN patients. Functionally, circ_0000064 promoted HG-induced proliferation, inflammation, cell cycle progression and ECM accumulation, while its knockdown alleviated these DN-related phenotypes. Mechanistically, circ_0000064 acted as a miR-424-5p sponge to upregulate WNT2B, supporting the circ_0000064/miR-424-5p/WNT2B axis as a potential biomarker or therapeutic mechanism in DN.
Confidence score:

0.7591
Other information
Title:

Circ_0000064 knockdown attenuates high glucose-induced proliferation, inflammation and extracellular matrix deposition of mesangial cells through miR-424-5p-mediated WNT2B inhibition in cell models of diabetic nephropathy.

Journal: Clinical and experimental nephrology
Published: 2022
PubMed ID: 35678923
Study type:

combined biological and clinical study
Data availability: The analyzed data sets generated during the present study are available from the corresponding author on reasonable request; https:// doi. org/ 10. 1007/ s10157-022-02241-w
Code availability: -