circRNA basic information
circBase ID: hsa_circ_0069616
Name: hsa_circ_ATP8A1
Synonym: circATP8A1 / hsa_circRNA_0069616
Host Gene: ATP8A1
Genomic location(hg19): chr4:42545933-42627730:-
Genomic location(hg38): chr4:42543916-42625713:-
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
0001056
MONDO name: gastric cancer
Disease details: gastric cancer / GC
Disease DO ID:
10534
Disease MeSH ID:
-
Disease NCIt ID:
C9331
Disease ICD11 ID:
1397617262
Disease OMIM ID:
613659
Species: Human
Species details: Homo sapiens
Tissue specimen:

gastric cancer tissue; plasma exosomes

Cell lines:

AGS; SGC-7901; HGC-27; MKN-45; GES-1; HEK-293 T; THP-1

In vivo animal model:

cell line-derived xenograft

circRNA-disease information
Expression pattern:
UP
Associated gene: STAT6, AGO2
Associated microRNA: miR-1-3p
Biological function: Promotes gastric cancer proliferation, migration, invasion, tumor growth, and induces macrophage M2 polarization via exosomes.
Molecular mechanism: Exosomal circATP8A1 acts as a ceRNA to sponge miR-1-3p, thereby relieving miR-1-3p-mediated repression of STAT6 and activating the STAT6 pathway to drive macrophage M2 polarization, promoting gastric cancer progression.
Biological pathway or process:

ceRNA regulation (promotes); immune regulation (promotes); migration (promotes); invasion (promotes); proliferation (promotes); other pathway/process (promotes)

Detected method:
Q
M
Validation methods:

Microarray; RT-qPCR; Clinical Sample Validation; Cohort Study; Survival Analysis; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RNase R Treatment; Actinomycin D / DRB Stability Assay; FISH / smFISH; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Western Blot; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); ELISA

Clinical significance:

Higher circATP8A1 expression is associated with advanced TNM stage and worse prognosis/poor survival in gastric cancer patients; elevated in plasma exosomes, suggesting prognostic biomarker potential.

Description:

circATP8A1 is upregulated in gastric cancer tissues and plasma exosomes and promotes gastric cancer progression. It is delivered via exosomes to macrophages, where it sponges miR-1-3p to activate STAT6 signaling, inducing M2 polarization that enhances tumor growth and migration; high circATP8A1 is associated with worse prognosis.

Confidence score:

0.8884

Other information
Title:

Exosome circATP8A1 induces macrophage M2 polarization by regulating the miR-1-3p/STAT6 axis to promote gastric cancer progression.

Journal: Molecular cancer
Published: 2024
PubMed ID: 38459596
Study type:

combined biological and clinical study

Data availability: GSE183904
Code availability: -