| Expression pattern: |
UP |
| Associated gene: |
HuR, SREBP1 mRNA, Myc, SREBP1 promoter |
| Associated microRNA: |
- |
| Biological function: |
promotes TNBC cell proliferation and invasion; enhances lipogenesis and fatty acid metabolism reprogramming; promotes tumor growth in vivo |
| Molecular mechanism: |
circMyc binds HuR in cytoplasm to enhance HuR binding to SREBP1 mRNA 3'-UTR and increase SREBP1 mRNA stability; circMyc binds Myc in nucleus to enhance Myc occupancy on SREBP1 promoter and increase SREBP1 transcription |
| Biological pathway or process: |
lipid metabolism (promotes); proliferation (promotes); invasion (promotes); mRNA stability (promotes); other pathway/process (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; RNA-seq; Bioinformatics Analysis; Transfection; RNA Pull-Down; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; ChIP / ChIP-seq; Western Blot; CCK8; EdU Staining; Transwell Assay; In Vivo Animal Model; Clinical Sample Validation; Cohort Study; Survival Analysis; IHC (Immunohistochemistry) |
| Clinical significance: |
High circMyc was closely related to larger tumor volume, later clinical stage and lymph node metastasis, and patients with high circMyc had an adverse overall survival time. |
| Description: |
circMyc (hsa_circ_0085533) is up-regulated in TNBC and promotes proliferation, invasion, lipogenesis and tumor growth. Mechanistically, cytoplasmic circMyc binds HuR to stabilize SREBP1 mRNA, while nuclear circMyc binds Myc to enhance Myc occupancy at the SREBP1 promoter and increase SREBP1 transcription, driving fatty acid metabolism reprogramming. |
| Confidence score: |
0.8669 |