| Expression pattern: |
UP |
| Associated gene: |
IGF2BP3, JAK/STAT3 pathway, JAK/STAT pathway |
| Associated microRNA: |
miR-214-3p |
| Biological function: |
Promotes LUAD cell proliferation, invasion, metastasis, EMT, tumor growth, and TAM M2 polarization; inhibits apoptosis and TAM M1 polarization. |
| Molecular mechanism: |
Exosomal circ_0051799 acts through a ceRNA mechanism by competitively adsorbing miR-214-3p, thereby regulating IGF2BP3 expression and activating the IGF2BP3-mediated JAK/STAT3 pathway. |
| Biological pathway or process: |
JAK/STAT (promotes); proliferation (promotes); invasion (promotes); metastasis (promotes); EMT (promotes); apoptosis (inhibits); immune regulation (promotes); macrophage polarization (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
H
M
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Actinomycin D / DRB Stability Assay; RT-qPCR; Microarray; FISH / smFISH; Clinical Sample Validation; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Annexin V/PI Flow Cytometry; Transwell Assay; Wound Healing Assay; TUNEL; In Vivo Animal Model; H&E Staining; ELISA; Western Blot; IHC (Immunohistochemistry); IF (Immunofluorescence); Bioinformatics Analysis |
| Clinical significance: |
circ_0051799 is highly expressed in LUAD tumor samples and LUAD cells and is more elevated in more severe clinical stages; the authors propose it may provide a molecular marker basis for early LUAD screening and targeted therapy. |
| Description: |
hsa_circ_0051799 is up-regulated in LUAD tissues, LUAD cells, and hypoxia-induced EVs. It promotes LUAD proliferation, invasion, metastasis, EMT, and TAM M2 polarization while inhibiting apoptosis, mainly through an exosomal circ_0051799/miR-214-3p/IGF2BP3 axis that activates the JAK/STAT3 pathway. |
| Confidence score: |
0.8686 |