circRNA basic information
circBase ID: hsa_circ_0085535
Name: hsa_circ_MYC
Synonym: circREOS
Host Gene: MYC
Genomic location(hg19): chr8:128750493-128753680:+
Genomic location(hg38): chr8:127738247-127741434:+
Subcellular localization: nucleus
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0009807
MONDO name: osteosarcoma
Disease details: osteosarcoma / OS
Disease DO ID:
 3347
Disease MeSH ID:
 D012516
Disease NCIt ID:
 C9145
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

-
Cell lines:

hFOB 1.19; 143B; HOS; SJSA-1; MG-63
In vivo animal model:

-
circRNA-disease information
Expression pattern:
DN
Associated gene: HuR, MYC, FASN, AGO2
Associated microRNA: -
Biological function: Suppresses lipid metabolism/synthesis, proliferation, growth, and invasion in osteosarcoma cells.
Molecular mechanism: circREOS binds the RBP HuR and restrains HuR binding/activation on the MYC mRNA 3'-UTR, reducing MYC and downstream MYC-facilitated FASN regulation, thereby lowering lipid accumulation.
Biological pathway or process:

lipid metabolism (inhibits); proliferation (inhibits); invasion (inhibits); migration (other); ceRNA regulation (other)
Detected method:
Q
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; RT-qPCR; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; RIP (RNA Immunoprecipitation); Actinomycin D / DRB Stability Assay; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Western Blot; Bioinformatics Analysis
Clinical significance:

-
Description:

circREOS (hsa_circ_0085535), a MYC-derived circRNA, is down-regulated and mainly nuclear in osteosarcoma cells. It suppresses MYC expression by binding HuR and limiting HuR-mediated activation/stabilization of MYC mRNA, thereby reducing FASN-driven lipid accumulation and inhibiting OS cell proliferation and invasion.
Confidence score:

0.7986
Other information
Title:

CircREOS suppresses lipid synthesis and osteosarcoma progression through inhibiting HuR-mediated MYC activation.

Journal: Journal of Cancer
Published: 2023
PubMed ID: 37151387
Study type:

biological research
Data availability: GSE42352
Code availability: -