| Expression pattern: |
DN |
| Associated gene: |
SRSF3, FN1 |
| Associated microRNA: |
- |
| Biological function: |
Suppresses NSCLC cell proliferation, cell cycle progression, migration, invasion, tumor growth and metastasis. |
| Molecular mechanism: |
Binds the RBP SRSF3 to down-regulate FN1 expression (SRSF3/FN1 axis). |
| Biological pathway or process: |
proliferation (inhibits); cell cycle (inhibits); migration (inhibits); invasion (inhibits); metastasis (inhibits) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; divergent primers PCR; RNase R Treatment; Sanger Sequencing; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Transfection; Colony Formation Assay; Cell Cycle Assay; Annexin V/PI Flow Cytometry; Transwell Assay; RNA Pull-Down; RIP (RNA Immunoprecipitation); Western Blot; RNA-seq; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); Clinical Sample Validation; Bioinformatics Analysis |
| Clinical significance: |
Decreased expression is associated with larger primary tumor size and advanced/worse clinical stage; potential biomarker and therapeutic target. |
| Description: |
hsa_circ_0050386 is down-regulated in NSCLC tissues and cell lines and acts as a tumor suppressor. It inhibits NSCLC proliferation, cell-cycle progression, migration/invasion, tumor growth and lung metastasis by binding the RBP SRSF3 and reducing FN1 expression (SRSF3/FN1 axis). Decreased hsa_circ_0050386 levels are associated with larger tumor size and more advanced clinical stage. |
| Confidence score: |
0.8671 |