| Expression pattern: |
UP |
| Associated gene: |
CXCR4, VEGF-A, Cyclin D1, N-cadherin, E-cadherin |
| Associated microRNA: |
miR-206 |
| Biological function: |
promotes CRC cell proliferation, migration and angiogenesis; high expression is associated with tumor size, TNM stage, lymph node metastasis, distant metastasis and poor overall survival |
| Molecular mechanism: |
circ_0056618 functions through a ceRNA mechanism by sponging miR-206, relieving miR-206-mediated repression and upregulating CXCR4 and VEGF-A |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); angiogenesis (promotes); ceRNA regulation (promotes); metastasis (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Transfection; CCK8; Transwell Assay; Tube Formation Assay; Western Blot; Luciferase Reporter Assay; Bioinformatics Analysis; Survival Analysis; Cohort Study |
| Clinical significance: |
circ_0056618 high expression is associated with poor overall survival and with tumor size, TNM stage, lymph node metastasis and distant metastasis; it might be a prognostic marker and potential therapeutic target for CRC |
| Description: |
circ_0056618 is up-regulated in human colorectal cancer tissues and CRC cell lines, and high expression is associated with metastasis-related clinicopathological features and poor overall survival. Functionally, circ_0056618 promotes CRC cell proliferation, migration and angiogenesis. Mechanistically, it sponges miR-206 and upregulates CXCR4 and VEGF-A, suggesting a circ_0056618/miR-206/CXCR4-VEGF-A regulatory axis and potential prognostic or therapeutic value in CRC. |
| Confidence score: |
0.7396 |