| Expression pattern: |
UP |
| Associated gene: |
YWHAZ, ASCT2, GLS1 |
| Associated microRNA: |
miR-149-5p |
| Biological function: |
promotes migration, invasion, glutaminolysis and tumor growth |
| Molecular mechanism: |
acts as a ceRNA by sponging miR-149-5p to regulate YWHAZ expression |
| Biological pathway or process: |
migration (promotes); invasion (promotes); glutaminolysis (promotes); proliferation (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Transfection; Transwell Assay; Western Blot; RNA Pull-Down; Luciferase Reporter Assay; In Vivo Animal Model; Bioinformatics Analysis |
| Clinical significance: |
high circNHSL1 expression was associated with tumor size, TNM stages, lymphatic metastasis, and distant metastasis; proposed as a potential biomarker for prognosis |
| Description: |
circNHSL1 is up-regulated in gastric cancer tissues, cells, and serum/cell-derived exosomes. It promotes GC migration, invasion, glutaminolysis and in vivo tumor growth by acting as a ceRNA that sponges miR-149-5p to increase YWHAZ expression. |
| Confidence score: |
0.7759 |