| Expression pattern: |
UP |
| Associated gene: |
DNMT1, IL-6 |
| Associated microRNA: |
- |
| Biological function: |
Promotes NSCLC cell proliferation, migration and invasion; modulates cell cycle; inhibits apoptosis; mediates osimertinib resistance; promotes tumor growth in vivo. |
| Molecular mechanism: |
Binds DNMT1 (identified by RNA pull-down and mass spectrometry) and regulates IL-6, forming a circRBM33/DNMT1/IL-6 axis contributing to osimertinib resistance. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); cell cycle (promotes); drug resistance (promotes); PI3K/AKT (other) |
| Detected method: |
Q
S
|
| Validation methods: |
RNA-seq; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; Transfection; CCK8; Annexin V/PI Flow Cytometry; Cell Cycle Assay; Transwell Assay; RNA Pull-Down; Western Blot; In Vivo Animal Model; Bioinformatics Analysis |
| Clinical significance: |
CircRBM33 is proposed as a promising diagnostic and prognostic marker for NSCLC. |
| Description: |
CircRBM33 (hsa_circ_0001772; host gene RBM33) is upregulated in NSCLC models of osimertinib resistance (H1975-OR). Its knockdown suppresses proliferation, migration/invasion, tumor growth and drug resistance while promoting apoptosis, potentially by binding DNMT1 and influencing the DNMT1/IL-6 axis. |
| Confidence score: |
0.6742 |