| Expression pattern: |
UP |
| Associated gene: |
PKM2, ALKBH5 |
| Associated microRNA: |
miR-541-5p, miR-3064-5p |
| Biological function: |
promotes glycolysis; promotes cell proliferation; promotes tumorigenesis |
| Molecular mechanism: |
circNRIP1 acts as a ceRNA sponge for miR-541-5p and miR-3064-5p to upregulate PKM2; ALKBH5-mediated m6A modification regulates circNRIP1 stability/expression |
| Biological pathway or process: |
glycolysis (promotes); proliferation (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; Bioinformatics Analysis; RT-qPCR; RNase R Treatment; Sanger Sequencing; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; FISH / smFISH; Western Blot; IHC (Immunohistochemistry); Luciferase Reporter Assay; MeRIP / MeRIP-seq; Transfection; CCK8; In Vivo Animal Model; H&E Staining |
| Clinical significance: |
Elevated circNRIP1 levels in PTC patients were correlated with higher TNM stage and larger tumor size; circNRIP1 can be a prognostic biomarker and therapeutic target for PTC |
| Description: |
circNRIP1 is up-regulated in papillary thyroid cancer and mainly localizes to the cytoplasm. It promotes glycolysis and proliferation/tumor growth by sponging miR-541-5p and miR-3064-5p to increase PKM2 expression, and its expression/stability is regulated via ALKBH5-dependent m6A modification. |
| Confidence score: |
0.8375 |