circRNA basic information
circBase ID: hsa_circ_0011167
Name: hsa_circ_EPB41
Synonym: circEPB41(2)
Host Gene: EPB41
Genomic location(hg19): chr1:29313942-29314417:+
Genomic location(hg38): chr1:28987430-28987905:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0007256
MONDO name: hepatocellular carcinoma
Disease details: hepatocellular carcinoma
Disease DO ID:
 684, 686
Disease MeSH ID:
 D006528
Disease NCIt ID:
 C3099
Disease ICD11 ID:
 1294035808
Disease OMIM ID:
 114550
Species: Human
Species details: Homo sapiens
Tissue specimen:

liver tissue; HCC tissue; adjacent normal tissue; xenograft tumors
Cell lines:

HepG2; PLC; Huh7; THLE-3
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: FTO, SIRT6 mRNA, HNRNPA1, SREBP-1, ChREBP, CEBPA, FASN, ACC1, ACLY, SCD, SCD1
Associated microRNA: -
Biological function: Promotes de novo lipid synthesis, lipid accumulation, lipogenic gene expression, HCC cell proliferation, colony formation, and xenograft tumor growth; inhibits apoptosis under glucose deprivation conditions.
Molecular mechanism: Glucose deprivation induces circEPB41(2) through HNRNPA1-dependent alternative splicing; circEPB41(2) interacts with FTO and enhances FTO demethylase activity, reducing SIRT6 mRNA stability, increasing H3K9ac/H3K27ac levels, and activating lipogenic gene expression.
Biological pathway or process:

other pathway/process (promotes); proliferation (promotes); apoptosis (inhibits); mRNA stability (inhibits); m6A modification (other)
Detected method:
Q
S
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Actinomycin D / DRB Stability Assay; RT-qPCR; RNA-seq; FISH / smFISH; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; ChIP / ChIP-seq; MeRIP / MeRIP-seq; Transfection; Colony Formation Assay; Annexin V/PI Flow Cytometry; In Vivo Animal Model; Oil Red O Staining; Western Blot; Cohort Study; Survival Analysis; Bioinformatics Analysis
Clinical significance:

circEPB41(2) was elevated in HCC tissues, positively associated with lipogenesis genes, and high circEPB41(2) expression was associated with poor patient prognosis and worse overall survival.
Description:

circEPB41(2), also reported as circ_0011167/circ_NO.13, is a human EPB41-derived circRNA up-regulated in HCC and induced by glucose deprivation through HNRNPA1-dependent splicing. It promotes HCC lipogenesis and tumor growth by interacting with FTO, enhancing FTO demethylase activity, destabilizing SIRT6 mRNA, and increasing H3K9ac/H3K27ac-dependent lipogenic gene expression. Clinically, elevated circEPB41(2) is associated with poorer overall survival in patients with HCC.
Confidence score:

0.895
Other information
Title:

Energy Stress-Induced circEPB41(2) Promotes Lipogenesis in Hepatocellular Carcinoma.

Journal: Cancer research
Published: 2025
PubMed ID: 39636740
Study type:

combined biological and clinical study
Data availability: GSE234825; Supplementary Datasets S2 and S3; Supplementary Dataset S1; Supplementary Dataset S4; Supplementary Dataset S5
Code availability: -