| Expression pattern: |
DN |
| Associated gene: |
ELAVL1, GALNT3 mRNA, QKI mRNA, GALNT3, QKI, E-cadherin |
| Associated microRNA: |
- |
| Biological function: |
suppresses RCC cell migration and metastasis; suppresses mesenchymal state/EMT; suppresses angiogenesis and vascular permeability |
| Molecular mechanism: |
Acts as an RBP scaffold/ternary complex: circSPIRE1 binds ELAVL1 and guides ELAVL1 binding to GALNT3 and QKI mRNAs to stabilize them, upregulating GALNT3 and QKI; GALNT3 promotes O-GalNAc glycosylation and membrane localization of E-cadherin; QKI promotes circSPIRE1 biogenesis via flanking introns forming a positive feedback loop; circSPIRE1 is packaged into exosomes and transferred to endothelial cells to suppress permeability and angiogenesis |
| Biological pathway or process: |
migration (inhibits); invasion (inhibits); metastasis (inhibits); EMT (inhibits); angiogenesis (inhibits); ceRNA regulation (inhibits) |
| Detected method: |
Q
B
S
|
| Validation methods: |
RNA-seq; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RNase R Treatment; Actinomycin D / DRB Stability Assay; Northern Blot; Nuclear-Cytoplasmic Fractionation; FISH / smFISH; RNA Pull-Down; RIP (RNA Immunoprecipitation); Transfection; Transwell Assay; Wound Healing Assay; Luciferase Reporter Assay; Western Blot; IF (Immunofluorescence); IHC (Immunohistochemistry); Tube Formation Assay; In Vivo Animal Model; H&E Staining; Bioinformatics Analysis; Survival Analysis; ROC Analysis |
| Clinical significance: |
Lower expression correlates with poorer DFS; may predict metastasis; improves DFS predictive ability of SSIGN scoring scale; downregulated in sera of metastatic patients |
| Description: |
circSPIRE1 (hsa_circ_0000829), derived from SPIRE1, is downregulated in metastatic RCC and acts as a metastasis suppressor. It functions mainly as a cytoplasmic/exosomal RNA scaffold: binding ELAVL1 to enhance stabilization of GALNT3 and QKI mRNAs, thereby promoting E-cadherin glycosylation/membrane localization and maintaining an epithelial state; QKI feeds back to promote circSPIRE1 biogenesis. Exosomal circSPIRE1 also suppresses endothelial permeability and angiogenesis, and higher circSPIRE1 associates with better DFS. |
| Confidence score: |
0.8941 |