| Expression pattern: |
UP |
| Associated gene: |
KDM4C, HMGA2, ADAM19, BMP4, AGO2 |
| Associated microRNA: |
miR-874, miR-1292 |
| Biological function: |
Promotes CRC cell proliferation, migration, invasion and liver metastasis; promotes EMT-related metastasis |
| Molecular mechanism: |
Nuclear circPABPC1 recruits KDM4C to HMGA2 promoter to reduce H3K9me3 and activate transcription; cytoplasmic circPABPC1 acts as a miRNA sponge (miR-874/miR-1292) to protect ADAM19 and BMP4 from miRNA-mediated degradation |
| Biological pathway or process: |
EMT (promotes); proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); apoptosis (inhibits); JAK/STAT (other); ceRNA regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RNA-seq; Bioinformatics Analysis; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; Colony Formation Assay; Transwell Assay; Western Blot; ChIP / ChIP-seq; In Vivo Animal Model; IHC (Immunohistochemistry) |
| Clinical significance: |
CircPABPC1 may serve as a novel biomarker and antimetastatic therapeutic target in CRC (including serum exosomal circPABPC1). |
| Description: |
circPABPC1 is up-regulated in CRC tissues and serum/cell-derived exosomes and promotes CRC proliferation and liver metastasis. It functions in the nucleus by recruiting KDM4C to activate HMGA2 transcription, and in the cytoplasm as a ceRNA sponging miR-874/miR-1292 to upregulate ADAM19 and BMP4, thereby promoting EMT-associated metastasis. |
| Confidence score: |
0.8817 |