circRNA basic information
circBase ID: hsa_circ_0069117
Name: hsa_circ_TBC1D14
Synonym: -
Host Gene: TBC1D14
Genomic location(hg19): chr4:7006570-7016284:+
Genomic location(hg38): chr4:7004843-7014557:+
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0009807
MONDO name: osteosarcoma
Disease details: osteosarcoma / OS
Disease DO ID:
 3347
Disease MeSH ID:
 D012516
Disease NCIt ID:
 C9145
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

osteosarcoma tissues (OST); paired adjacent normal tissues
Cell lines:

U2OS; 143B; MG63; HOS; hMSCs; 293 T
In vivo animal model:

-
circRNA-disease information
Expression pattern:
DN
Associated gene: PF4V1, ERK1, AKT
Associated microRNA: miR-875-3p
Biological function: Suppresses proliferation and migration of osteosarcoma cells
Molecular mechanism: Acts as a miRNA sponge for miR-875-3p to increase PF4V1 expression, leading to suppression of ERK1 and AKT expression/phosphorylation
Biological pathway or process:

proliferation (inhibits); migration (inhibits); ERK (inhibits); PI3K/AKT (inhibits); ceRNA regulation (promotes)
Detected method:
Q
M
Validation methods:

Microarray; Bioinformatics Analysis; RT-qPCR; Clinical Sample Validation; Luciferase Reporter Assay; Transfection; Western Blot; CCK8; Wound Healing Assay
Clinical significance:

-
Description:

In osteosarcoma, hsa_circ_0069117 is down-regulated and functions as a tumor-suppressive circRNA. It sponges miR-875-3p to increase PF4V1, which in turn suppresses ERK1 and AKT signaling, leading to reduced proliferation and migration of OS cells.
Confidence score:

0.6777
Other information
Title:

Circular RNA hsa_circ_0069117 suppresses proliferation and migration of osteosarcoma cells lines via miR-875-3p/PF4V1 axis.

Journal: Journal of orthopaedic surgery and research
Published: 2022
PubMed ID: 35062989
Study type:

combined biological and clinical study
Data availability: GSE96964; GSE70367; All data generated during this study are available from the corresponding author.
Code availability: -