| Expression pattern: |
UP |
| Associated gene: |
ELAVL1, SCD mRNA, SCD |
| Associated microRNA: |
- |
| Biological function: |
Promotes CRC cell proliferation, migration and tumorigenicity, and protects CRC cells against ferroptosis by reducing lipid peroxidation and ferroptotic cell death. |
| Molecular mechanism: |
circRHBDD1 acts as a scaffold to mediate the interaction between ELAVL1 and SCD mRNA, enhancing SCD mRNA stability and promoting SCD expression, thereby suppressing ferroptosis and promoting CRC progression. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); ferroptosis (inhibits); mRNA stability (promotes); lipid metabolism (other); other pathway/process (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RNase R Treatment; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Transfection; CCK8; Colony Formation Assay; Transwell Assay; RIP (RNA Immunoprecipitation); RNA Pull-Down; Western Blot; IHC (Immunohistochemistry); In Vivo Animal Model; ROC Analysis; Bioinformatics Analysis |
| Clinical significance: |
circRHBDD1 expression in serum is associated with CRC advancement and liver metastasis and has potential as a diagnostic marker for CRC. |
| Description: |
circRHBDD1 is a human RHBDD1-derived circRNA that is upregulated in colorectal cancer tissues, serum and cell lines. It promotes CRC proliferation, migration and xenograft tumorigenicity while repressing ferroptosis by scaffolding ELAVL1 and SCD mRNA to enhance SCD mRNA stability and SCD expression. Its serum expression is associated with CRC progression and liver metastasis and shows diagnostic potential. |
| Confidence score: |
0.8812 |