| Expression pattern: |
UP |
| Associated gene: |
STAT3, FUS, p-STAT3 |
| Associated microRNA: |
miR-296-5p |
| Biological function: |
promotes proliferation, migration and invasion; promotes tumor growth and metastasis |
| Molecular mechanism: |
Acts as a miR-296-5p sponge to upregulate STAT3; interacts with FUS to facilitate nuclear translocation of p-STAT3 |
| Biological pathway or process: |
JAK/STAT (promotes); proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Bioinformatics Analysis; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Sanger Sequencing; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); RNA Pull-Down; Co-IP; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Western Blot; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); Microarray |
| Clinical significance: |
- |
| Description: |
circRPPH1 is up-regulated in bladder cancer cells and promotes proliferation, migration/invasion and in vivo tumor growth/metastasis. It functions as a ceRNA by sponging miR-296-5p to increase STAT3, and also binds the RBP FUS to facilitate nuclear transport of p-STAT3, thereby enhancing STAT3 signaling. |
| Confidence score: |
0.8626 |