| Expression pattern: |
UP |
| Associated gene: |
FOXM1, Slug |
| Associated microRNA: |
miR-149 |
| Biological function: |
promotes colorectal cancer cell migration and invasion |
| Molecular mechanism: |
Acts as a ceRNA/miRNA sponge for miR-149 to increase FOXM1 expression and modulate FOXM1/Slug protein levels. |
| Biological pathway or process: |
migration (promotes); invasion (promotes); EMT (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RT-qPCR; Clinical Sample Validation; Transfection; Wound Healing Assay; Transwell Assay; Luciferase Reporter Assay; Western Blot; Bioinformatics Analysis |
| Clinical significance: |
Increased circANKS1B expression was significantly associated with regional lymph node metastasis and distance metastasis. |
| Description: |
circANKS1B is up-regulated in colorectal cancer tissues and cell lines and promotes CRC cell migration and invasion. Mechanistically, it functions as a miR-149 sponge to increase FOXM1 expression and elevate FOXM1/Slug levels, thereby enhancing metastatic phenotypes. |
| Confidence score: |
0.7129 |