circRNA basic information
circBase ID: hsa_circ_0079440
Name: hsa_circ_PHF14
Synonym: circPHF14
Host Gene: PHF14
Genomic location(hg19): chr7:11101417-11151094:+
Genomic location(hg38): chr7:11061790-11111467:+
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0001056
MONDO name: gastric cancer
Disease details: gastric cancer / GC
Disease DO ID:
 10534
Disease MeSH ID:
 -
Disease NCIt ID:
 C9331
Disease ICD11 ID:
 1397617262
Disease OMIM ID:
 613659
Species: Human
Species details: Homo sapiens
Tissue specimen:

-
Cell lines:

AGS; 7901; GES1
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: DDX5
Associated microRNA: -
Biological function: Promotes gastric cancer cell proliferation, growth, migration, and tumorigenesis; circPHF14 silencing reverses DDX5-induced tumor-promoting effects.
Molecular mechanism: DDX5 induces circPHF14 expression; circPHF14 mediates DDX5-driven gastric cancer progression. miRNA sponge mechanism was proposed but not demonstrated.
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (other); metastasis (other)
Detected method:
Q
S
Validation methods:

circRNA-seq; RT-qPCR; RNase R Treatment; Sanger Sequencing; Actinomycin D / DRB Stability Assay; Transfection; CCK8; EdU Staining; Colony Formation Assay; Transwell Assay; In Vivo Animal Model
Clinical significance:

-
Description:

This study identifies circPHF14 (hsa_circ_0079440), derived from PHF14, as a DDX5-induced circRNA that promotes gastric cancer cell proliferation, migration, and xenograft tumor growth. Silencing circPHF14 partially reverses the tumor-promoting effects caused by DDX5 overexpression, indicating circPHF14 acts as a key downstream effector of DDX5 in gastric cancer.
Confidence score:

0.6458
Other information
Title:

RNA helicase DDX5-induced circPHF14 promotes gastric cancer cell progression.

Journal: Aging
Published: 2023
PubMed ID: 36996491
Study type:

biological research
Data availability: GSE207636
Code availability: -