circRNA basic information
circBase ID: hsa_circ_0000251
Name: hsa_circ_IDE
Synonym: circIDE
Host Gene: IDE
Genomic location(hg19): chr10:94291504-94297307:-
Genomic location(hg38): chr10:92531747-92537550:-
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
0007256
MONDO name: hepatocellular carcinoma
Disease details: hepatocellular carcinoma / HCC
Disease DO ID:
684, 686
Disease MeSH ID:
D006528
Disease NCIt ID:
C3099
Disease ICD11 ID:
1294035808
Disease OMIM ID:
114550
Species: Human
Species details: Homo sapiens
Tissue specimen:

HCC tissues; tumour-adjacent tissues / adjacent liver tissues

Cell lines:

HepG2; Hep3B

In vivo animal model:

syngeneic model

circRNA-disease information
Expression pattern:
DN
Associated gene: RBMS1, GPX4
Associated microRNA: miR-19b-3p
Biological function: Enhances ferroptosis and inhibits proliferation/tumour growth in HCC.
Molecular mechanism: Acts as a miRNA sponge for miR-19b-3p to elevate RBMS1, leading to decreased GPX4 and increased ferroptosis.
Biological pathway or process:

ferroptosis (promotes); proliferation (inhibits); ceRNA regulation (promotes)

Detected method:
Q
Validation methods:

RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; FISH / smFISH; Luciferase Reporter Assay; RNA Pull-Down; Transfection; Western Blot; EdU Staining; In Vivo Animal Model; IHC (Immunohistochemistry); Survival Analysis; Bioinformatics Analysis; Clinical Sample Validation

Clinical significance:

High circIDE expression is associated with better OS and RFS; low circIDE expression corresponds with unfavourable survival in HCC.

Description:

circIDE (hsa_circ_0000251), derived from the IDE gene, is down-regulated in HCC and mainly localized in the cytoplasm. It sponges miR-19b-3p to increase RBMS1, thereby reducing GPX4 and promoting ferroptosis, which suppresses HCC cell proliferation and tumour growth; higher circIDE is associated with better survival.

Confidence score:

0.8633

Other information
Title:

Suppressing circIDE/miR-19b-3p/RBMS1 axis exhibits promoting-tumour activity through upregulating GPX4 to diminish ferroptosis in hepatocellular carcinoma.

Journal: Epigenetics
Published: 2023
PubMed ID: 36989117
Study type:

combined biological and clinical study

Data availability: GSE155949; GSE156088
Code availability: -