circRNA basic information
circBase ID: -
Name: hsa_circ_XPO1
Synonym: circXPO1 / hsa_circ_102737
Host Gene: XPO1
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0018177
MONDO name: glioblastoma
Disease details: glioblastoma / GBM
Disease DO ID:
 3068
Disease MeSH ID:
 D005909
Disease NCIt ID:
 C3058
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

glioma tissues; normal brain tissues
Cell lines:

U87-MG; U251; 293T; HA1800; NHA
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: RAF1
Associated microRNA: miR-7-5p
Biological function: promotes GBM cell proliferation and migration; promotes gliomagenesis in vivo
Molecular mechanism: ceRNA sponging of miR-7-5p to de-repress RAF1 expression
Biological pathway or process:

proliferation (promotes); migration (promotes); cell cycle (promotes); ceRNA regulation (promotes)
Detected method:
Q
M
Validation methods:

Microarray; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Actinomycin D / DRB Stability Assay; FISH / smFISH; Luciferase Reporter Assay; Transfection; CCK8; BrdU; Cell Cycle Assay; Wound Healing Assay; IF (Immunofluorescence); In Vivo Animal Model; IHC (Immunohistochemistry); Survival Analysis; Bioinformatics Analysis
Clinical significance:

circXPO1 expression was positively correlated with the tumor-bearing survival
Description:

circXPO1 is up-regulated in glioma/GBM tissues and cell lines and promotes proliferation, cell-cycle progression, migration, and in vivo tumor growth. Mechanistically, it acts as a ceRNA that sponges miR-7-5p, leading to increased RAF1 expression and enhanced GBM malignancy.
Confidence score:

0.8458
Other information
Title:

CircXPO1 Promotes Glioblastoma Malignancy by Sponging miR-7-5p.

Journal: Cells
Published: 2023
PubMed ID: 36980172
Study type:

combined biological and clinical study
Data availability: GSE146463
Code availability: -