| Expression pattern: |
DN |
| Associated gene: |
Fragile X mental retardation protein (FMRP), human antigen R (HuR) |
| Associated microRNA: |
- |
| Biological function: |
regulates dendritic spine morphology and maturation; reduced circ_Satb1 decreases branched-like (complex) dendritic spines |
| Molecular mechanism: |
circ_Satb1 downregulation (CRISPR/CasRx knockdown) alters dendritic spine morphology; potential interaction with RBPs (FMRP, HuR) suggested by CircInteractome |
| Biological pathway or process: |
ceRNA regulation (other); other pathway/process (other) |
| Detected method: |
Q
S
|
| Validation methods: |
RNA-seq; RT-qPCR; RNase R Treatment; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; FISH / smFISH; Clinical Sample Validation; Bioinformatics Analysis; Transfection; IF (Immunofluorescence) |
| Clinical significance: |
circ_Satb1 expression was positively correlated with the seizure-free postoperative period in mTLE non-HS, suggesting high circ_Satb1 expression may be a predictor of a positive postoperative outcome |
| Description: |
circ_Satb1 (SATB1-derived) is down-regulated in the hippocampus of mTLE patients and also decreased in a chronic-stage kainate mouse model. CRISPR/CasRx-mediated circ_Satb1 knockdown in primary hippocampal neurons reduces complex/branched dendritic spines, indicating a role in dendritic spine morphology/maturation and potential relevance to epileptogenesis. circ_Satb1 levels correlate positively with postoperative seizure-free period, suggesting possible predictive value for surgical outcome. |
| Confidence score: |
0.7331 |