circRNA basic information
circBase ID: hsa_circ_0000650
Name: hsa_circ_SEMA4B
Synonym: circSEMA4B
Host Gene: SEMA4B
Genomic location(hg19): chr15:90760670-90764997:+
Genomic location(hg38): chr15:90217438-90221765:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0007254
MONDO name: breast cancer
Disease details: breast cancer / BC
Disease DO ID:
 1612
Disease MeSH ID:
 -
Disease NCIt ID:
 C9335
Disease ICD11 ID:
 1047754165
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

tumor tissues; adjacent normal tissues
Cell lines:

MDA-MB-231; HCC-1937; BT549; MCF-7; SKBR3; MCF-10A; HEK293T
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
DN
Associated gene: PDCD4, AKT, PI3K/AKT signaling pathway, AGO2
Associated microRNA: miR-330-3p
Biological function: inhibits proliferation; inhibits migration; inhibits invasion; suppresses tumor growth
Molecular mechanism: Encodes SEMA4B-211aa via IRES-dependent translation and acts as a miRNA sponge (miR-330-3p) to upregulate PDCD4, inhibiting AKT phosphorylation (Ser473) and PI3K/AKT signaling.
Biological pathway or process:

PI3K/AKT (inhibits); proliferation (inhibits); migration (inhibits); invasion (inhibits); ceRNA regulation (promotes)
Detected method:
Q
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; MTT; Colony Formation Assay; Wound Healing Assay; Transwell Assay; Western Blot; Co-IP; GST pull-down assay; ELISA; In Vivo Animal Model; IHC (Immunohistochemistry)
Clinical significance:

Low expression of circSEMA4B was positively associated with TNM stage, tumor size, lymph node metastasis, recurrence, and metastasis.
Description:

circSEMA4B is down-regulated in breast cancer and functions as a tumor suppressor. It inhibits PI3K/AKT signaling by sponging miR-330-3p to upregulate PDCD4 (reducing AKT Ser473 phosphorylation) and also serves as a protein-coding circRNA that produces SEMA4B-211aa, which contributes to pathway suppression.
Confidence score:

0.8752
Other information
Title:

CircSEMA4B inhibits the progression of breast cancer by encoding a novel protein SEMA4B-211aa and regulating AKT phosphorylation.

Journal: Cell death & disease
Published: 2022
PubMed ID: 36115854
Study type:

combined biological and clinical study
Data availability: The PI3K/AKT signaling-associated genes were obtained from PathCards (https://pathcards.genecards.org/). The circRNAs sequence data were obtained from circbase (http://circrna.org/). All data included in this study are available upon request by contact with the corresponding author.
Code availability: -