| Expression pattern: |
UP |
| Associated gene: |
CTCF, HOXB1, PDE4D |
| Associated microRNA: |
- |
| Biological function: |
promotes migration and invasion; promotes metastasis; induces EMT |
| Molecular mechanism: |
circSTK3 is transcriptionally regulated by CTCF and promotes CRC metastasis by inducing EMT programming; associated with metastasis-related gene-expression changes including HOXB1 and PDE4D modulation. |
| Biological pathway or process: |
migration (promotes); invasion (promotes); metastasis (promotes); EMT (promotes); Hippo/YAP (other) |
| Detected method: |
Q
H
M
|
| Validation methods: |
Microarray; RT-qPCR; RNase R Treatment; ISH (In Situ Hybridization); Clinical Sample Validation; Survival Analysis; Transfection; Transwell Assay; Wound Healing Assay; CCK8; Cell Cycle Assay; Western Blot; IF (Immunofluorescence); ChIP / ChIP-seq; In Vivo Animal Model; H&E Staining; Bioinformatics Analysis |
| Clinical significance: |
Upregulation/high expression of circSTK3 is associated with poor prognosis and decreased survival; circSTK3 is an independent prognostic factor in CRC. |
| Description: |
circSTK3 (hsa_circ_0004592), derived from STK3, is up-regulated in colorectal cancer tissues and is mainly localized in the cytoplasm. Functional assays show it promotes CRC cell migration, invasion, and in vivo lung metastasis by inducing EMT programs, and its high expression predicts poor overall survival; CTCF transcriptionally promotes circSTK3 expression. |
| Confidence score: |
0.7956 |