| Expression pattern: |
UP |
| Associated gene: |
HLA-I, HLA-II, HLA-A2, CD4+ T cells, CD8+ T cells, dendritic cells, circFAM53B-219, circFAM53B(192-200), circFAM53B(210-218), circFAM53B(181-219) |
| Associated microRNA: |
- |
| Biological function: |
Encodes cryptic antigenic peptides that prime antigen-specific CD4+ and CD8+ T cells, enhance cytotoxic T-cell infiltration, induce anti-tumour immunity, suppress tumour growth in PDX models, and are associated with better prognosis; circFAM53B did not influence tumour-cell proliferation, apoptosis, migration, invasion or EMT in breast cancer cells. |
| Molecular mechanism: |
Noncanonical translation of circFAM53B produces circFAM53B-219 and HLA-binding cryptic antigenic peptides, including circFAM53B(192-200) and circFAM53B(210-218), which bind HLA/MHC molecules and stimulate antigen-specific adaptive anti-tumour immunity. |
| Biological pathway or process: |
immune regulation (promotes); apoptosis (promotes); proliferation (inhibits); metastasis (inhibits); other pathway/process (promotes) |
| Detected method: |
Q
B
H
S
|
| Validation methods: |
RNase R Treatment; Sanger Sequencing; RT-qPCR; RNA-seq; Northern Blot; FISH / smFISH; ISH (In Situ Hybridization); IHC (Immunohistochemistry); IF (Immunofluorescence); Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Luciferase Reporter Assay; Transfection; CCK8; Annexin V/PI Flow Cytometry; Transwell Assay; TUNEL; In Vivo Animal Model; Western Blot; Cohort Study; Survival Analysis; Bioinformatics Analysis; Flow Cytometry（Non-apoptosis/cycle） |
| Clinical significance: |
Higher circFAM53B RNA and circFAM53B-219 peptide expression correlated with higher infiltration of circFAM53B-specific CTLs, smaller tumour size and better patient survival in breast cancer and melanoma; circFAM53B RNA was an independent prognostic factor for breast cancer outcomes. |
| Description: |
Human circFAM53B is a tumour-specific circRNA derived from FAM53B that encodes cryptic antigenic peptides presented by HLA molecules. These peptides activate antigen-specific CD4+ and CD8+ T-cell responses, suppress tumour growth in breast cancer PDX models, and high circFAM53B/circFAM53B-219 expression is linked to better prognosis in breast cancer and melanoma. |
| Confidence score: |
0.8541 |