circRNA basic information
circBase ID: hsa_circ_0000517
Name: -
Synonym: circ_0000517
Host Gene: -
Genomic location(hg19): chr14:20811404-20811492:-
Genomic location(hg38): chr14:20343245-20343333:-
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
0007256
MONDO name: hepatocellular carcinoma
Disease details: hepatocellular carcinoma
Disease DO ID:
684, 686
Disease MeSH ID:
D006528
Disease NCIt ID:
C3099
Disease ICD11 ID:
1294035808
Disease OMIM ID:
114550
Species: Human
Species details: Homo sapiens
Tissue specimen:

tumor tissue; adjacent nontumor tissues

Cell lines:

THLE-2; HCCLM3; Huh7

In vivo animal model:

cell line-derived xenograft

circRNA-disease information
Expression pattern:
UP
Associated gene: IGF1R
Associated microRNA: miR-326
Biological function: circ_0000517 promotes hepatocellular carcinoma cell viability/proliferation, colony formation, migration, invasion and glycolysis; circ_0000517 knockdown suppresses these malignant phenotypes and reduces xenograft tumor growth.
Molecular mechanism: ceRNA mechanism: circ_0000517 sponges miR-326, thereby relieving miR-326-mediated repression of IGF1R.
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); glycolysis (promotes); ceRNA regulation (promotes); metastasis (other)

Detected method:
Q
Validation methods:

RNase R Treatment; RT-qPCR; Clinical Sample Validation; Bioinformatics Analysis; Luciferase Reporter Assay; Transfection; MTT; Colony Formation Assay; Transwell Assay; Western Blot; In Vivo Animal Model

Clinical significance:

High expression of circ_0000517 in hepatocellular carcinoma; previous study reported that upregulation of circ_0000517 indicated poor outcomes.

Description:

circ_0000517 is up-regulated in hepatocellular carcinoma and promotes malignant phenotypes (proliferation/viability, migration, invasion and glycolysis). Mechanistically, it acts as a ceRNA that sponges miR-326 to increase IGF1R expression, and circ_0000517 knockdown suppresses tumor growth in a Huh7 xenograft model.

Confidence score:

0.7177

Other information
Title:

Circular RNA circ_0000517 regulates hepatocellular carcinoma development via miR-326/IGF1R axis.

Journal: Cancer cell international
Published: 2020
PubMed ID: 32863763
Study type:

combined biological and clinical study

Data availability: The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.
Code availability: -