| Expression pattern: |
UP |
| Associated gene: |
SMAD2, EIF4A3 |
| Associated microRNA: |
miR-7 |
| Biological function: |
facilitates tumorigenesis and progression of NSCLC by promoting proliferation, migration and invasion (metastasis/EMT-related changes). |
| Molecular mechanism: |
EIF4A3 promotes circSCAP expression; circSCAP acts as a ceRNA by sponging miR-7 to upregulate SMAD2. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); EMT (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Bioinformatics Analysis; RT-qPCR; Nuclear-Cytoplasmic Fractionation; RNase R Treatment; Actinomycin D / DRB Stability Assay; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); RNA Pull-Down; Transfection; CCK8; Wound Healing Assay; Transwell Assay; Western Blot; Clinical Sample Validation; Survival Analysis; Microarray |
| Clinical significance: |
circSCAP expression was inversely correlated with the overall survival rate of NSCLC patients; positively correlated with tumor size, cell differentiation, lymph node metastasis, and distant metastasis. |
| Description: |
circSCAP is up-regulated in NSCLC tissues and cell lines and mainly localizes to the cytoplasm. It promotes NSCLC cell proliferation, migration and invasion by acting as a ceRNA that sponges miR-7, thereby de-repressing SMAD2; its high expression is associated with worse overall survival. |
| Confidence score: |
0.8625 |