| Expression pattern: |
UP |
| Associated gene: |
YTHDF1, PIK3R1, EIF4A3, GLUT1, HK2, PI3K/AKT |
| Associated microRNA: |
- |
| Biological function: |
promotes HCC cell proliferation and aerobic glycolysis; promotes tumor growth; restricts anti-PD-1 therapy efficacy |
| Molecular mechanism: |
circRHBDD1 acts as a scaffold recruiting the m6A reader YTHDF1 to PIK3R1 mRNA to enhance PIK3R1 translation in an m6A-dependent manner; EIF4A3 promotes circRHBDD1 biogenesis via exon back-splicing; activation of PI3K/AKT signaling |
| Biological pathway or process: |
glycolysis (promotes); proliferation (promotes); PI3K/AKT (promotes); m6A modification (promotes) |
| Detected method: |
Q
S
|
| Validation methods: |
circRNA-seq; RT-qPCR; RNase R Treatment; Sanger Sequencing; divergent primers PCR; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Actinomycin D / DRB Stability Assay; RNA-seq; Clinical Sample Validation; Survival Analysis; Transfection; RIP (RNA Immunoprecipitation); CLIP; RNA Pull-Down; Western Blot; Luciferase Reporter Assay; MeRIP / MeRIP-seq; CCK8; EdU Staining; Colony Formation Assay; In Vivo Animal Model; IHC (Immunohistochemistry); IF (Immunofluorescence); Bioinformatics Analysis |
| Clinical significance: |
high circRHBDD1 expression was correlated with poor overall survival and disease-free survival; circRHBDD1 level was an independent prognostic factor for overall survival and disease-free survival |
| Description: |
circRHBDD1 is up-regulated in HCC and promotes proliferation and glycolysis. It acts as a scaffold to recruit YTHDF1 to PIK3R1 mRNA, enhancing m6A-dependent translation of PIK3R1 and activating PI3K/AKT signaling, thereby promoting tumor growth and reducing anti-PD-1 therapy efficacy. |
| Confidence score: |
0.9002 |