circRNA basic information
circBase ID: -
Name: -
Synonym: hsa_circ_001653
Host Gene: -
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0001056
MONDO name: gastric cancer
Disease details: gastric cancer
Disease DO ID:
 10534
Disease MeSH ID:
 -
Disease NCIt ID:
 C9331
Disease ICD11 ID:
 1397617262
Disease OMIM ID:
 613659
Species: Human
Species details: Homo sapiens
Tissue specimen:

GC tissues; adjacent normal tissues; tumor tissues; serum
Cell lines:

SGC-7901; MKN-45; MGC80-3; MKN-28; GES-1
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: NR6A1, Ago2, PCNA, Ki67, MMP2, MMP9, cleaved caspase 3, cleaved caspase 9
Associated microRNA: miR-377
Biological function: hsa_circ_001653 promotes gastric cancer cell proliferation, migration, invasion and tumor growth, and inhibits apoptosis.
Molecular mechanism: hsa_circ_001653 acts through a ceRNA-like mechanism by competitively binding miR-377, thereby increasing NR6A1 expression.
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); ceRNA regulation (other)
Detected method:
Q
M
Validation methods:

Microarray; RT-qPCR; Clinical Sample Validation; Bioinformatics Analysis; Transfection; CCK8; Annexin V/PI Flow Cytometry; Transwell Assay; Western Blot; FISH / smFISH; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); In Vivo Animal Model; ELISA
Clinical significance:

High hsa_circ_001653 expression is associated with advanced TNM stage, deep tumor infiltration, lymph node metastasis and distant metastasis in gastric cancer patients.
Description:

hsa_circ_001653 is up-regulated in gastric cancer tissues and cell lines and is associated with more aggressive clinicopathological features. Functionally, it promotes gastric cancer cell proliferation, migration, invasion and xenograft tumor growth while inhibiting apoptosis. Mechanistically, cytoplasmic hsa_circ_001653 binds miR-377 and increases NR6A1 expression, forming a hsa_circ_001653/miR-377/NR6A1 regulatory axis.
Confidence score:

0.742
Other information
Title:

hsa_circ_001653 up-regulates NR6A1 expression and elicits gastric cancer progression by binding to microRNA-377.

Journal: Experimental physiology
Published: 2020
PubMed ID: 33006200
Study type:

combined biological and clinical study
Data availability: GSE83521; GSE19826; GSE65801; TCGA
Code availability: -