circRNA basic information
circBase ID: hsa_circ_0000740
Name: hsa_circ_DHX33
Synonym: circDHX33
Host Gene: DHX33
Genomic location(hg19): chr17:5364258-5365866:-
Genomic location(hg38): chr17:5460938-5462546:-
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0008315
MONDO name: prostate cancer
Disease details: prostate cancer
Disease DO ID:
 10283
Disease MeSH ID:
 D011471
Disease NCIt ID:
 C7378
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

PCa tissues; adjacent normal tissues
Cell lines:

RWPE-1; 22Rv1; PC-3; DU145
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
DN
Associated gene: IGF2BP2
Associated microRNA: -
Biological function: CircDHX33 suppresses glycolytic activity, proliferation, migration, invasion, EMT, tumor growth, and metastasis, and promotes apoptosis in PCa.
Molecular mechanism: CircDHX33 directly interacts with the RNA-binding protein IGF2BP2 and reduces IGF2BP2 protein stability/expression without affecting IGF2BP2 mRNA levels, thereby inhibiting malignant PCa behaviors.
Biological pathway or process:

glycolysis (inhibits); proliferation (inhibits); migration (inhibits); invasion (inhibits); EMT (inhibits); apoptosis (promotes); metastasis (inhibits); other pathway/process (inhibits)
Detected method:
Q
M
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; FISH / smFISH; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Transfection; EdU Staining; Colony Formation Assay; Annexin V/PI Flow Cytometry; Transwell Assay; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); Western Blot; Bioinformatics Analysis
Clinical significance:

Low CircDHX33 expression is associated with TNM stage and distant metastasis in PCa, and CircDHX33 may serve as a biomarker and therapeutic target.
Description:

CircDHX33 is down-regulated in prostate cancer tissues and cell lines. It functions as a tumor-suppressive circRNA by binding the RNA-binding protein IGF2BP2, reducing IGF2BP2 protein stability/expression, and thereby inhibiting glycolysis, proliferation, migration, invasion, EMT, tumor growth, and metastasis while promoting apoptosis. Low circDHX33 expression is associated with advanced TNM stage and distant metastasis, supporting its potential biomarker and therapeutic relevance.
Confidence score:

0.8571
Other information
Title:

circDHX33 suppresses glycolysis, malignant proliferation, and metastasis in prostate cancer by interacting with RNA-binding protein IGF2BP2 to destabilize its protein.

Journal: Cytotechnology
Published: 2025
PubMed ID: 39927135
Study type:

combined biological and clinical study
Data availability: GSE113153; GEPIA; available from the corresponding author on reasonable request
Code availability: -