| Expression pattern: |
UP |
| Associated gene: |
KLF9 |
| Associated microRNA: |
miR-93-5p |
| Biological function: |
Promotes inflammation, oxidative stress and apoptosis in AKI; circHIPK3 knockdown alleviates kidney injury and improves renal function. |
| Molecular mechanism: |
ceRNA mechanism: circHIPK3 sponges miR-93-5p, relieving repression of KLF9, thereby promoting AKI-associated inflammation/oxidative stress/apoptosis. |
| Biological pathway or process: |
inflammation (promotes); oxidative phosphorylation (inhibits); apoptosis (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Luciferase Reporter Assay; Transfection; MTT; Annexin V/PI Flow Cytometry; ELISA; Western Blot; In Vivo Animal Model; H&E Staining; TUNEL; Bioinformatics Analysis |
| Clinical significance: |
- |
| Description: |
circHIPK3 is up-regulated in renal I/R-induced AKI and in H/R-treated HK-2 cells. It aggravates renal tubular cell injury by sponging miR-93-5p to increase KLF9, thereby promoting inflammation, oxidative stress and apoptosis; circHIPK3 knockdown alleviates AKI in vitro and in vivo. |
| Confidence score: |
0.5637 |