| Expression pattern: |
DN |
| Associated gene: |
Notch1mRNA / NOTCH1 mRNA |
| Associated microRNA: |
miR-129-5p |
| Biological function: |
Inhibits carcinogenesis; suppresses cell proliferation; regulates cell cycle transition |
| Molecular mechanism: |
Acts as a miR-129-5p sponge (ceRNA) to regulate NOTCH1 mRNA expression |
| Biological pathway or process: |
ceRNA regulation (other); proliferation (inhibits); cell cycle (inhibits) |
| Detected method: |
Q
M
|
| Validation methods: |
divergent primers PCR; RT-qPCR; Sanger Sequencing; Luciferase Reporter Assay; Transfection; Cell Cycle Assay; In Vivo Animal Model; Bioinformatics Analysis; Microarray |
| Clinical significance: |
Low expression of hsa_circ_0005986 was correlated with chronic hepatitis B family history, tumor diameters, microvascular invasion, and BCLC stage; might be a potential biomarker for the diagnosis of HCC |
| Description: |
In hepatocellular carcinoma, hsa_circ_0005986 is down-regulated in tumors and HCC cell lines. It acts as a ceRNA sponge for miR-129-5p to modulate NOTCH1 mRNA, and its knockdown promotes cell-cycle progression and proliferation. Clinically, low hsa_circ_0005986 expression correlates with multiple aggressive clinicopathological features and is proposed as a diagnostic biomarker. |
| Confidence score: |
0.7082 |