| Expression pattern: |
DN |
| Associated gene: |
HOXA7, Ago2, MMP2, MMP9 |
| Associated microRNA: |
miR-3127-5p |
| Biological function: |
CircPAPPA supports trophoblast cell proliferation, migration, invasion and cell cycle progression, and inhibits apoptosis; circPAPPA silencing suppresses these biological functions and may contribute to PE pathogenesis. |
| Molecular mechanism: |
CircPAPPA acts as a ceRNA that targets miR-3127-5p to positively regulate HOXA7 expression, thereby regulating trophoblast cell biological functions in PE. |
| Biological pathway or process: |
ceRNA regulation (other); proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); cell cycle (promotes) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; MTT; Colony Formation Assay; Annexin V/PI Flow Cytometry; Cell Cycle Assay; Transwell Assay; Western Blot; Bioinformatics Analysis |
| Clinical significance: |
CircPAPPA might be a potential biomarker and therapeutic target for PE; low circPAPPA expression was associated with higher systolic blood pressure, higher diastolic blood pressure, higher proteinuria, and lower infant body weight in PE patients. |
| Description: |
CircPAPPA is down-regulated in placental tissues from PE patients and is mainly cytoplasmic in trophoblast cells. It promotes trophoblast proliferation, migration, invasion and cell cycle progression while suppressing apoptosis through the miR-3127-5p/HOXA7 ceRNA axis, suggesting biomarker and therapeutic-target potential for PE. |
| Confidence score: |
0.8278 |