circRNA basic information
circBase ID: -
Name: hsa_circ_DUSP16
Synonym: circDUSP16
Host Gene: DUSP16
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005580
MONDO name: esophageal squamous cell carcinoma
Disease details: esophageal squamous cell carcinoma
Disease DO ID:
 3748
Disease MeSH ID:
 C562729; D000077277
Disease NCIt ID:
 C4024
Disease ICD11 ID:
 1417891145
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

ESCC tissues
Cell lines:

-
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: TKTL1
Associated microRNA: miR-497-5p
Biological function: CircDUSP16 promotes hypoxia-induced ESCC cell viability, colony formation, migration, invasion, glycolysis, and tumorigenesis; knockdown of circDUSP16 suppresses these malignant phenotypes.
Molecular mechanism: circDUSP16 positively regulates TKTL1 expression by sponging miR-497-5p in ESCC cells.
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); glycolysis (promotes); ceRNA regulation (promotes)
Detected method:
Q
Validation methods:

RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; Clinical Sample Validation; RNA Pull-Down; Luciferase Reporter Assay; CCK8; Colony Formation Assay; Transwell Assay; Western Blot; In Vivo Animal Model
Clinical significance:

-
Description:

circDUSP16 is up-regulated in ESCC tissues, ESCC cells, and hypoxia-stimulated ESCC cells. Functionally, circDUSP16 promotes ESCC cell viability, colony formation, migration, invasion, glycolysis, and in vivo tumorigenesis. Mechanistically, it acts through a circDUSP16/miR-497-5p/TKTL1 regulatory axis by sponging miR-497-5p to positively regulate TKTL1 expression.
Confidence score:

0.7782
Other information
Title:

CircDUSP16 Contributes to Cell Development in Esophageal Squamous Cell Carcinoma by Regulating miR-497-5p/TKTL1 Axis.

Journal: The Journal of surgical research
Published: 2021
PubMed ID: 33326930
Study type:

combined biological and clinical study
Data availability: -
Code availability: -