circRNA basic information
circBase ID: hsa_circ_0004214
Name: hsa_circ_AMOTL1
Synonym: circ0004214 / CircAMOTL1
Host Gene: AMOTL1
Genomic location(hg19): chr11:94532555-94533477:+
Genomic location(hg38): chr11:94799389-94800311:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0021042
MONDO name: glioma
Disease details: glioma
Disease DO ID:
 -
Disease MeSH ID:
 D005910
Disease NCIt ID:
 C3059
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

glioma tissue; paracancerous tissue; non-glioma control brain tissue / control cortex
Cell lines:

U87
In vivo animal model:

-
circRNA-disease information
Expression pattern:
UP
Associated gene: RBP
Associated microRNA: miR-28-3p, miR-379-5p, miR-382-5p, miR-370-3p
Biological function: promotes migration of glioma cells
Molecular mechanism: Predicted ceRNA interaction network with miRNAs and RBPs (not experimentally validated).
Biological pathway or process:

migration (promotes); ceRNA regulation (other)
Detected method:
Q
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RT-qPCR; FISH / smFISH; Transfection; Transwell Assay; Bioinformatics Analysis; Microarray; ROC Analysis; Clinical Sample Validation
Clinical significance:

Diagnostic biomarker for glioma and especially high-grade glioma (WHO III/IV) with AUC 0.880 and 0.931.
Description:

hsa_circ_0004214 (host gene AMOTL1) is up-regulated in glioma tissues, especially in high-grade gliomas (WHO III/IV), and shows strong diagnostic performance by ROC analysis. Functionally, overexpression enhances and knockdown reduces U87 cell migration. A putative ceRNA/RBP interaction network involving miR-28-3p, miR-379-5p, miR-382-5p, and miR-370-3p was bioinformatically predicted but not fully validated.
Confidence score:

0.717
Other information
Title:

A Meaningful Strategy for Glioma Diagnosis via Independent Determination of hsa_circ_0004214.

Journal: Brain sciences
Published: 2023
PubMed ID: 36831736
Study type:

combined biological and clinical study
Data availability: GSE13030; GEO: GSE146463; Supplementary Material File S1; Supplementary Material File S2
Code availability: -