| Expression pattern: |
UP |
| Associated gene: |
Snail1, Twist1 |
| Associated microRNA: |
miR-153 |
| Biological function: |
Induces EMT and metastasis; promotes migration and invasion of laryngeal cancer cells; hsa-circ_0058106 knockdown inhibits migration, invasion, EMT, and lung metastasis. |
| Molecular mechanism: |
hsa-circ_0058106 acts as a miR-153 sponge to regulate Snail1 expression and interacts with Twist1 to induce Twist1 nuclear translocation. |
| Biological pathway or process: |
EMT (promotes); metastasis (promotes); migration (promotes); invasion (promotes); ceRNA regulation (promotes); other pathway/process (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RNase R Treatment; RT-qPCR; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Luciferase Reporter Assay; RNA Pull-Down; RIP (RNA Immunoprecipitation); Transfection; Wound Healing Assay; Transwell Assay; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); Western Blot; IF (Immunofluorescence); Bioinformatics Analysis |
| Clinical significance: |
High hsa-circ_0058106 expression is associated with lymph node metastasis and advanced clinical stages, suggesting it may be related to laryngeal cancer metastasis and may serve as a potential therapeutic target. |
| Description: |
hsa-circ_0058106 is upregulated in laryngeal cancer tissues and its high expression is associated with lymph node metastasis and advanced clinical stage. Functionally, it promotes laryngeal cancer cell migration, invasion, EMT, and in vivo metastasis. Mechanistically, it acts as a miR-153 sponge to regulate Snail1 expression and interacts with Twist1 to promote Twist1 nuclear translocation. |
| Confidence score: |
0.8399 |