circRNA basic information
circBase ID: hsa_circ_0006646
Name: hsa_circ_PTK2
Synonym: circ_0006646
Host Gene: PTK2
Genomic location(hg19): chr8:141889569-141900868:-
Genomic location(hg38): chr8:140879470-140890769:-
Subcellular localization: cytoplasm; exosome
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
0002974
MONDO name: cervical cancer
Disease details: Cervical cancer / CC
Disease DO ID:
4362
Disease MeSH ID:
-
Disease NCIt ID:
C9311
Disease ICD11 ID:
1256072522
Disease OMIM ID:
603956
Species: Human
Species details: Homo sapiens
Tissue specimen:

plasma; exosomes; tumor tissue

Cell lines:

SiHa; CaSki; C33A; HeLa; Ect1/E6E7

In vivo animal model:

cell line-derived xenograft

circRNA-disease information
Expression pattern:
UP
Associated gene: RRM2
Associated microRNA: miR-758-3p
Biological function: promotes cervical cancer cell growth, proliferation, migration, invasion, EMT, metastasis and tumor growth; inhibits apoptosis
Molecular mechanism: circ_0006646 acts as a sponge for miR-758-3p to upregulate RRM2 expression, thereby promoting cervical cancer progression
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); EMT (promotes); apoptosis (inhibits); ceRNA regulation (promotes)

Detected method:
Q
Validation methods:

RT-qPCR; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Bioinformatics Analysis; Transfection; CCK8; EdU Staining; Annexin V/PI Flow Cytometry; Transwell Assay; Western Blot; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); RNA Pull-Down; In Vivo Animal Model; IHC (Immunohistochemistry); ROC Analysis

Clinical significance:

exosomal circ_0006646 might be a potential diagnostic indicator of cervical cancer; AUC value was 0.8691

Description:

circ_0006646 is up-regulated in cervical cancer cells and in plasma exosomes from cervical cancer patients. It promotes cervical cancer growth, proliferation, migration, invasion, EMT, metastasis and in vivo tumor growth by sponging miR-758-3p and increasing RRM2 expression. Exosomal circ_0006646 showed diagnostic potential for distinguishing cervical cancer patients from healthy controls.

Confidence score:

0.8146

Other information
Title:

Circ_0006646 Accelerates the Growth and Metastasis of Cervical Cancer by Elevating RRM2 Through miR-758-3p.

Journal: Biochemical genetics
Published: 2023
PubMed ID: 36583788
Study type:

combined biological and clinical study

Data availability: The datasets used and analyzed during the current study are available from the corresponding author on reasonable request.
Code availability: -