circRNA basic information
circBase ID: hsa_circ_0006950
Name: hsa_circ_TRPS1
Synonym: circ-TRPS1
Host Gene: TRPS1
Genomic location(hg19): chr8:116631358-116635985:-
Genomic location(hg38): chr8:115619131-115623758:-
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0008315
MONDO name: prostate cancer
Disease details: prostate cancer / PCa
Disease DO ID:
 10283
Disease MeSH ID:
 D011471
Disease NCIt ID:
 C7378
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

PCa tissues; H-PCa tissues; L-PCa tissues; tumor tissues
Cell lines:

PC3; LnCaP; DU145; RWPE-1; HEK293T
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: EZH2, OCT4, Nanog, Ki67
Associated microRNA: miR-124-3p
Biological function: circ-TRPS1 promotes PCa proliferation, migration, invasion, metastasis, and cancer stem-like cell differentiation; circ-TRPS1 knockdown suppresses these malignant phenotypes.
Molecular mechanism: circ-TRPS1 functions as a miR-124-3p sponge to enhance EZH2 expression, thereby promoting EZH2-mediated stemness and PCa progression.
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); stemness (promotes); ceRNA regulation (other); other pathway/process (promotes)
Detected method:
Q
H
S
Validation methods:

RNase R Treatment; circRNA-seq; RT-qPCR; FISH / smFISH; Clinical Sample Validation; Bioinformatics Analysis; Luciferase Reporter Assay; RNA Pull-Down; Transfection; CCK8; Colony Formation Assay; Transwell Assay; In Vivo Animal Model; IHC (Immunohistochemistry); Western Blot
Clinical significance:

High circ-TRPS1 expression correlated with aggressive PCa phenotypes and unfavorable prognosis; circ-TRPS1 may be a prognostic/predictive biomarker and candidate therapeutic target.
Description:

circ-TRPS1 is up-regulated in high-grade prostate cancer tissues and PCa cell lines, and high expression is associated with aggressive clinicopathologic features and poor prognosis. Functionally, circ-TRPS1 promotes PCa proliferation, migration, invasion, metastasis, and cancer stem-like cell differentiation by sponging miR-124-3p and increasing EZH2 expression. Its knockdown suppresses tumor growth and metastasis in vitro and in vivo, supporting circ-TRPS1 as a potential therapeutic target.
Confidence score:

0.8439
Other information
Title:

Downregulation of circ-TRPS1 suppressed prostatic cancer prognoses by regulating miR-124-3p/EZH2 axis-mediated stemness.

Journal: American journal of cancer research
Published: 2020
PubMed ID: 33415005
Study type:

combined biological and clinical study
Data availability: Data for this work have been uploaded.
Code availability: -