| Expression pattern: |
UP |
| Associated gene: |
PRC2 complex, EZH2, SUZ12, EED |
| Associated microRNA: |
miR-214-3p |
| Biological function: |
promotes DEX-induced chondrocyte injury (decreased viability and increased apoptosis); inhibits M2 macrophage polarization; inhibits angiogenesis |
| Molecular mechanism: |
circCDR1as recruits PRC2 (EZH2/SUZ12/EED) to the miR-214-3p promoter, increasing H3K27me3 and epigenetically repressing miR-214-3p (indirect regulation rather than direct miRNA sponging). |
| Biological pathway or process: |
apoptosis (promotes); proliferation (inhibits); macrophage polarization (inhibits); angiogenesis (inhibits); other pathway/process (other) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); ChIP / ChIP-seq; RNA Pull-Down; Transfection; CCK8; Annexin V/PI Flow Cytometry; Flow Cytometry（Non-apoptosis/cycle）; Tube Formation Assay; Western Blot |
| Clinical significance: |
- |
| Description: |
circCDR1as is up-regulated in LCPD patient cartilage/serum/chondrocytes and negatively correlates with miR-214-3p. It promotes DEX-induced chondrocyte injury and suppresses M2 macrophage polarization and angiogenesis by recruiting PRC2 (EZH2/SUZ12/EED) to epigenetically repress miR-214-3p via H3K27me3. |
| Confidence score: |
0.7334 |