| Expression pattern: |
UP |
| Associated gene: |
HNRNPA1, Ago2 |
| Associated microRNA: |
miR-646 |
| Biological function: |
promotes migration and proliferation; affects apoptosis; promotes tumor growth in vivo |
| Molecular mechanism: |
ceRNA mechanism: circTIAM1 sponges miR-646 to derepress HNRNPA1 |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); apoptosis (other); ceRNA regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; Bioinformatics Analysis; RT-qPCR; Clinical Sample Validation; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RNase R Treatment; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Transwell Assay; Wound Healing Assay; RNA-seq; Western Blot; IHC (Immunohistochemistry); H&E Staining; In Vivo Animal Model; Survival Analysis |
| Clinical significance: |
high level of circTIAM1 expression was associated with unfavorable prognosis (higher tumor-necrosis-metastasis stage, higher proportion of lymph node metastases, and larger tumor sizes), indicating that circTIAM1 might be a novel prognostic biomarker for PTC |
| Description: |
circTIAM1 (hsa_circ_0061406) is up-regulated in papillary thyroid cancer tissues and cell lines and promotes PTC cell proliferation and migration and supports tumor growth in xenografts. It mainly functions as a ceRNA by sponging miR-646, thereby relieving miR-646-mediated repression of HNRNPA1. |
| Confidence score: |
0.8802 |